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qiasymphony dsp virus pathogen mini kit  (Qiagen)


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    Qiagen qiasymphony dsp virus pathogen mini kit
    Qiasymphony Dsp Virus Pathogen Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 635 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dsp+virus+kit/QIAsymphony+DSP+Virus%2FPathogen+Mini+Kit/pmc12936764-46-5-10
    Average 97 stars, based on 635 article reviews
    qiasymphony dsp virus pathogen mini kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Control:

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Endotracheal aspirates Obtained from intubated critically ill patients in ICU N/A Critical commercial assays Viral transport medium LMR1925VTM, Labomoderne https://www.labomoderne.com/gamme.kits-de- prelevement-nasopharynges-avec-ecouvillons-floquessteriles-et-milieu-de-transport-special-virus-vtm-tigesecable-plastique-tete-en-nylon-floque.LMR1925VTM.html MS2 RNA bacteriophage/IC1 RNA internal control bioMérieux R-GENE® ref. 71-110 https://www.biomerieux.fr/fr/diagnostic-clinique/ controle-interne-adn-r-gene-et-controle-interne-arn-r-gene DNase Life Technologies ®, Carlsbad, USA Cat # AM1907 Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen®. .. Qiagen Cat #62724 Whole Transcriptome Amplification WTA2, Sigma-Aldrich®, Darmstadt, Germany Cat #41121800 QIAquick spin columns Qiagen®, Hilden, Germany Cat #28104 MS2 qPCR R-GENE® ref. 71-110 Cat # 20043675 Nextera XT kit Illumina, San Diego, USA Cat #FC-131-1096 COVIDSeq Test Kit ® Illumina, San Diego, USA https://emea.illumina.com/products/by-type/ ivd-products/covidseq.html Deposited data Viral Metagenomic sequencing data from IBIS cohort This study PRJNA1110359 Viral Metagenomic sequencing data from PREV-HAP cohort This study PRJNA1132989 The 16S rRNA data of the IBIS and PREV-HAP cohort Montassier et al. 20233 PRJNA1109383 Software and algorithms SraHumanScrubber v2.2.1 Open-source software https://github.com/ncbi/sra-human-scrubber Cutadapt v4.6 Open-source software https://cutadapt.readthedocs.io/en/ v4.6/installation.html kraken2 v2.1.2 Open-source software https://github.com/DerrickWood/kraken2 Krakentools Open-source software https://github.com/jenniferlu717/KrakenTools SPAdes v.3.14.0 Open-source software https://github.com/ablab/spades cd-hit v4.7 Open-source software https://github.com/weizhongli/cdhit Diamond v2.1.6.160 Open-source software https://github.com/bbuchfink/diamond Burrows-Wheeler Aligner (BWA) software v0.7.17 Open-source software https://github.com/lh3/bwa Samtools Open-source software https://github.com/samtools LEfSe v1.1.2 Open-source software https://github.com/SegataLab/lefse PhaBOX2 v2.1.10 Open-source software https://phage.ee.cityu.edu.hk/ VIBRANT v1.2.1 Open-source software https://github.com/AnantharamanLab/VIBRANT TKNA v1.2.1 Open-source software https://github.com/CAnBioNet/TkNA FFTREES v1.9.0 Open-source software https://github.com/ndphillips/FFTrees R v 4.2.3 Open-source software https://www.r-project.org/ Lung Virome pipeline Open-source git https://doi.org/10.5281/zenodo.15497802 https://github.com/genepii/HAP2-IBIS-Virome e1 Cell Reports Medicine 6, 102289, September 16, 2025

    Virus:

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples Endotracheal aspirates Obtained from intubated critically ill patients in ICU N/A Critical commercial assays Viral transport medium LMR1925VTM, Labomoderne https://www.labomoderne.com/gamme.kits-de- prelevement-nasopharynges-avec-ecouvillons-floquessteriles-et-milieu-de-transport-special-virus-vtm-tigesecable-plastique-tete-en-nylon-floque.LMR1925VTM.html MS2 RNA bacteriophage/IC1 RNA internal control bioMérieux R-GENE® ref. 71-110 https://www.biomerieux.fr/fr/diagnostic-clinique/ controle-interne-adn-r-gene-et-controle-interne-arn-r-gene DNase Life Technologies ®, Carlsbad, USA Cat # AM1907 Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen®. .. Qiagen Cat #62724 Whole Transcriptome Amplification WTA2, Sigma-Aldrich®, Darmstadt, Germany Cat #41121800 QIAquick spin columns Qiagen®, Hilden, Germany Cat #28104 MS2 qPCR R-GENE® ref. 71-110 Cat # 20043675 Nextera XT kit Illumina, San Diego, USA Cat #FC-131-1096 COVIDSeq Test Kit ® Illumina, San Diego, USA https://emea.illumina.com/products/by-type/ ivd-products/covidseq.html Deposited data Viral Metagenomic sequencing data from IBIS cohort This study PRJNA1110359 Viral Metagenomic sequencing data from PREV-HAP cohort This study PRJNA1132989 The 16S rRNA data of the IBIS and PREV-HAP cohort Montassier et al. 20233 PRJNA1109383 Software and algorithms SraHumanScrubber v2.2.1 Open-source software https://github.com/ncbi/sra-human-scrubber Cutadapt v4.6 Open-source software https://cutadapt.readthedocs.io/en/ v4.6/installation.html kraken2 v2.1.2 Open-source software https://github.com/DerrickWood/kraken2 Krakentools Open-source software https://github.com/jenniferlu717/KrakenTools SPAdes v.3.14.0 Open-source software https://github.com/ablab/spades cd-hit v4.7 Open-source software https://github.com/weizhongli/cdhit Diamond v2.1.6.160 Open-source software https://github.com/bbuchfink/diamond Burrows-Wheeler Aligner (BWA) software v0.7.17 Open-source software https://github.com/lh3/bwa Samtools Open-source software https://github.com/samtools LEfSe v1.1.2 Open-source software https://github.com/SegataLab/lefse PhaBOX2 v2.1.10 Open-source software https://phage.ee.cityu.edu.hk/ VIBRANT v1.2.1 Open-source software https://github.com/AnantharamanLab/VIBRANT TKNA v1.2.1 Open-source software https://github.com/CAnBioNet/TkNA FFTREES v1.9.0 Open-source software https://github.com/ndphillips/FFTrees R v 4.2.3 Open-source software https://www.r-project.org/ Lung Virome pipeline Open-source git https://doi.org/10.5281/zenodo.15497802 https://github.com/genepii/HAP2-IBIS-Virome e1 Cell Reports Medicine 6, 102289, September 16, 2025

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target.
    Article Snippet: .. QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, https://doi.org/10.3390/pathogens15010071 Qiagen, Hilden, Germany), EZ1 Advanced (DSP Virus Kit, Qiagen, Germany), and Maxwell RSC (RSC Pathogen total nucleic acid kit, Promega, Madison, WI, USA). ..

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients.
    Article Snippet: .. The samples were then filtered through 0.45 μm filters and incubated with DNase (Life Technologies , Carlsbad, USA) for 90 min. Total nucleic acid extraction was performed on the Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen. .. After extraction, both RNA and DNA amplification were performed using a Whole Transcriptome Amplification kit (WTA2, Sigma-Aldrich, Darmstadt, Germany).

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients
    Article Snippet: .. The samples were then filtered through 0.45 μm filters and incubated with DNase (Life Technologies , Carlsbad, USA) for 90 min. Total nucleic acid extraction was performed on the Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen. .. After extraction, both RNA and DNA amplification were performed using a Whole Transcriptome Amplification kit (WTA2, Sigma-Aldrich, Darmstadt, Germany).

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target
    Article Snippet: .. QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, Qiagen, Hilden, Germany), EZ1 Advanced (DSP Virus Kit, Qiagen, Germany), and Maxwell RSC (RSC Pathogen total nucleic acid kit, Promega, Madison, WI, USA). ..

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target
    Article Snippet: .. EZ1 (DSP Virus Kit, Qiagen, Germany) and Maxwell (RSC Pathogen total nucleic acid kit, Promega, USA) systems used magnetic-bead-based extraction, whereas QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, Qiagen, Germany) performed silica spin-column-based purification. ..

    Article Title: Combined doravirine and islatravir cooperate to inhibit NRTI and NNRTI resistant HIV-1 in vitro.
    Article Snippet: .. For the amplification of the target region, viral RNA was 118 extracted from the bottom 0.4 mL following centrifugation of 1.5 mL of plasma at 20,000 xg 119 for 90 minutes, by using the EZ1 automatic system and the DSP Virus Kit (Qiagen) according to 120 the manufacturer's instructions. .. The reverse transcription and first-round PCR were performed 121 using the SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity (Invitrogen) 122 using the primers P210 (5’-ACCCTTCAGGAACAAATAGSATGGA-3’, HXB2 nucleotide coordinates 123 1513-1537) and P220 (5’-TTCTGCTATTAAGTCTTTTGMTGGGTCRTA-3’, HXB2 3504-3533).

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target.
    Article Snippet: .. EZ1 (DSP Virus Kit, Qiagen, Germany) and Maxwell (RSC Pathogen total nucleic acid kit, Promega, USA) systems used magnetic-bead-based extraction, whereas QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, Qiagen, Germany) performed silica spin-column-based purification. ..

    Incubation:

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients.
    Article Snippet: .. The samples were then filtered through 0.45 μm filters and incubated with DNase (Life Technologies , Carlsbad, USA) for 90 min. Total nucleic acid extraction was performed on the Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen. .. After extraction, both RNA and DNA amplification were performed using a Whole Transcriptome Amplification kit (WTA2, Sigma-Aldrich, Darmstadt, Germany).

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients
    Article Snippet: .. The samples were then filtered through 0.45 μm filters and incubated with DNase (Life Technologies , Carlsbad, USA) for 90 min. Total nucleic acid extraction was performed on the Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen. .. After extraction, both RNA and DNA amplification were performed using a Whole Transcriptome Amplification kit (WTA2, Sigma-Aldrich, Darmstadt, Germany).

    Extraction:

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients.
    Article Snippet: .. The samples were then filtered through 0.45 μm filters and incubated with DNase (Life Technologies , Carlsbad, USA) for 90 min. Total nucleic acid extraction was performed on the Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen. .. After extraction, both RNA and DNA amplification were performed using a Whole Transcriptome Amplification kit (WTA2, Sigma-Aldrich, Darmstadt, Germany).

    Article Title: Lung virome convergence precedes hospital-acquired pneumonia in intubated critically ill patients
    Article Snippet: .. The samples were then filtered through 0.45 μm filters and incubated with DNase (Life Technologies , Carlsbad, USA) for 90 min. Total nucleic acid extraction was performed on the Qiagen EZ1 Advanced XL Extractor using the DSP Virus Kit, Qiagen. .. After extraction, both RNA and DNA amplification were performed using a Whole Transcriptome Amplification kit (WTA2, Sigma-Aldrich, Darmstadt, Germany).

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target
    Article Snippet: .. EZ1 (DSP Virus Kit, Qiagen, Germany) and Maxwell (RSC Pathogen total nucleic acid kit, Promega, USA) systems used magnetic-bead-based extraction, whereas QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, Qiagen, Germany) performed silica spin-column-based purification. ..

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target.
    Article Snippet: .. EZ1 (DSP Virus Kit, Qiagen, Germany) and Maxwell (RSC Pathogen total nucleic acid kit, Promega, USA) systems used magnetic-bead-based extraction, whereas QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, Qiagen, Germany) performed silica spin-column-based purification. ..

    Purification:

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target
    Article Snippet: .. EZ1 (DSP Virus Kit, Qiagen, Germany) and Maxwell (RSC Pathogen total nucleic acid kit, Promega, USA) systems used magnetic-bead-based extraction, whereas QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, Qiagen, Germany) performed silica spin-column-based purification. ..

    Article Title: Comparative Evaluation of Automated Nucleic Acid Extraction Systems for DNA and RNA Viral Target.
    Article Snippet: .. EZ1 (DSP Virus Kit, Qiagen, Germany) and Maxwell (RSC Pathogen total nucleic acid kit, Promega, USA) systems used magnetic-bead-based extraction, whereas QIAcube (QIAamp Viral RNA Kit and QIAamp DNA Blood Kit, Qiagen, Germany) performed silica spin-column-based purification. ..

    Amplification:

    Article Title: Combined doravirine and islatravir cooperate to inhibit NRTI and NNRTI resistant HIV-1 in vitro.
    Article Snippet: .. For the amplification of the target region, viral RNA was 118 extracted from the bottom 0.4 mL following centrifugation of 1.5 mL of plasma at 20,000 xg 119 for 90 minutes, by using the EZ1 automatic system and the DSP Virus Kit (Qiagen) according to 120 the manufacturer's instructions. .. The reverse transcription and first-round PCR were performed 121 using the SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity (Invitrogen) 122 using the primers P210 (5’-ACCCTTCAGGAACAAATAGSATGGA-3’, HXB2 nucleotide coordinates 123 1513-1537) and P220 (5’-TTCTGCTATTAAGTCTTTTGMTGGGTCRTA-3’, HXB2 3504-3533).

    Centrifugation:

    Article Title: Combined doravirine and islatravir cooperate to inhibit NRTI and NNRTI resistant HIV-1 in vitro.
    Article Snippet: .. For the amplification of the target region, viral RNA was 118 extracted from the bottom 0.4 mL following centrifugation of 1.5 mL of plasma at 20,000 xg 119 for 90 minutes, by using the EZ1 automatic system and the DSP Virus Kit (Qiagen) according to 120 the manufacturer's instructions. .. The reverse transcription and first-round PCR were performed 121 using the SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity (Invitrogen) 122 using the primers P210 (5’-ACCCTTCAGGAACAAATAGSATGGA-3’, HXB2 nucleotide coordinates 123 1513-1537) and P220 (5’-TTCTGCTATTAAGTCTTTTGMTGGGTCRTA-3’, HXB2 3504-3533).

    Clinical Proteomics:

    Article Title: Combined doravirine and islatravir cooperate to inhibit NRTI and NNRTI resistant HIV-1 in vitro.
    Article Snippet: .. For the amplification of the target region, viral RNA was 118 extracted from the bottom 0.4 mL following centrifugation of 1.5 mL of plasma at 20,000 xg 119 for 90 minutes, by using the EZ1 automatic system and the DSP Virus Kit (Qiagen) according to 120 the manufacturer's instructions. .. The reverse transcription and first-round PCR were performed 121 using the SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity (Invitrogen) 122 using the primers P210 (5’-ACCCTTCAGGAACAAATAGSATGGA-3’, HXB2 nucleotide coordinates 123 1513-1537) and P220 (5’-TTCTGCTATTAAGTCTTTTGMTGGGTCRTA-3’, HXB2 3504-3533).



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